pclnrx retrovirus expression vector Search Results


pclnrx expression vector  (Novus Biologicals)
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Novus Biologicals pclnrx expression vector
Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector <t>pCLNRX</t> to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax <t>System,</t> <t>Imgenex).</t> CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.
Pclnrx Expression Vector, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pclnrx+retrovirus+expression+vector/pCLNRX+Retrovirus+Expression+Vector/pm19543243-30-19-22
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pclnrx retrovirus expression vector  (Novus Biologicals)
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pCLNRX Retrovirus Expression Vector
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Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector pCLNRX to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax System, Imgenex). CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.

Journal: Cancer gene therapy

Article Title: Enhancement of antitumor activity of gammaretrovirus carrying IL-12 gene through genetic modification of envelope targeting HER2 receptor: a promising strategy for bladder cancer therapy.

doi: 10.1038/cgt.2009.41

Figure Lengend Snippet: Figure 1 Schematic illustrations of the plasmid constructs. The coding region of the anti-erbB2scFv was inserted into retroviral packaging vector pCL-Eco-DTM that lacked the transmembrane subunit of the envelope to generate pCL-EcoscFv. The p35 and p40 subunits of IL-12 as well as the sequence of the IRES were cloned into retroviral expression vector pCLNRX to yield pCL-IL12. The pCL-LacZ and pCL-Eco plasmids served as the controls. pCLMFG- LacZ, pCLNRX, and pCL-Eco were from the Moloney murine leukemia virus (MoMLV)-based pCL Vector System (RetroMax System, Imgenex). CMV, cytomegalovirus immediate-early promo- ter; RU5, direct repeat and unique sequence at 50 region of MoMLV RNA; c, packaging signal; LacZ, Escherichia coli b-galactosidase gene; LTR, long terminal repeat of MoMLV; NeoR, neomycin phosphotransferase gene; RSV, Rous sarcoma virus promoter; IL12p40, IL-12 p40 cDNA; IRES, encephalomyocarditis virus (EMCV) internal ribosome entry site; IL12p35, IL-12 p35 cDNA; SU, surface glycoprotein; TM, transmembrane; GAG, gag gene; POL, pol gene; SP, signal peptide; RBD, receptor-binding domain; PRR, proline-rich region; C, carboxyl terminal domain; VH, cDNA for variable fragment of heavy chain; VL, cDNA for variable fragment of light chain.

Article Snippet: The PCR products were individually cloned into the pGEM-T easy vector (Promega, Madison, WI), and then transferred into the pCLNRX expression vector (Imgenex), yielding pCL-IL12 plasmid (Figure 1).

Techniques: Plasmid Preparation, Construct, Retroviral, Sequencing, Clone Assay, Expressing, Virus, Binding Assay